About BD LSRFortessa As a frequently referenced high-parameter cell analyzers, the BD LSRFortessa brand provides power, performance and consistency for your research. Designed to be affordable and expandable, BD LSRFortessa cell analyzers have the flexibility to support the growing needs of multicolor flow cytometry assays.

The LSR Fortessa uses BD FacsFlow as the sheath fluid. 20 L boxes of which are available in lab 1.47a (CIMR) and on the bench behind the machine (e6) Please contact Flow Core Unit staff if none are present

Laser Detector. Filter ( nm). Example Fluorophores. (AF= Alexa Fluor).

20 L boxes of which are available in lab 1.47a (CIMR) and on the bench behind the machine (e6) Please contact Flow Core Unit staff if none are present The sheath fluid sits on the left of the FACS Flow Supply cart underneath the bench. BD™ High Throughput Sampler (HTS) provides rapid, fully automated sample acquisition from microtiter plates. In high-throughput mode, the HTS option can speed through a 96-well plate in less than 15 minutes with less than 0.5% sample carryover from one well to the next. BD FACS LSRFortessa HTS Operation Manual 1 FACS LSRFortessa Standard Operation Protocol HTS Mode You are strongly recommended to adjust voltage for each channel and setup compensation with Tube Mode before plate mode acquisition! (Please refer to LSR Fortessa Standard Operation Protocol – Basic operation for Tube Mode) Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Immunoanalytics - Core Facility Geb. 90 Marchioninistr. 25 81377 München BD High Throughput Sampler User’s Guide For the BD LSRFortessa I. Introduction The BD High Throughput Sampler (HTS) is a high-speed sampling device used on the 5-Laser LSRFortessa located in Rm. 6006F of the RIA Division.

BD LSR II / LSR Fortessa . February 2020 .

The BD Fortessa cell analyzer provides the flexibility to support measurement of multicolor flow cytometry assays of up to 16 parameters at a time. The FCF is

Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig. A1). b.

The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays.

5. Crafthouse Informed design inspired by the streamlined aesthetic of Deco architecture.

4. Remove the sheath container lid. 5. Crafthouse Informed design inspired by the streamlined aesthetic of Deco architecture.
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PerCP-Cy5.5, PerCP & FITC) BD Fortessa The BD Fortessa cell analyzer provides the flexibility to support measurement of multicolor flow cytometry assays of up to 16 parameters at a time. The FCF is equipped with one BD LSRFortessa™ (Diagnostic Purpose) and a BD LSRFortessa™ X-20 (Research Purpose). SOP: LSR-Fortessa Instrument Start-up 1. Locate the green power button on the right hand side of the instrument; press to switch on the LSR-Fortessa. This will warm up the lasers and begin to pressurise the fluidic system.

BD LSR Fortessa.
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Configuration and Fluorophore. Guide for LSR Fortessa. Laser Detector. Filter ( nm). Example Fluorophores. (AF= Alexa Fluor). UV D. 379/28. BUV 395. UV C.

Disconnect the air line (green) and fluid line (Blue) from the sheath container. 3. Depressurize the sheath container by pulling up on the vent valve. 4.

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Instruments & Services. Instruments & Services. Main Content. BD LSR Fortessa. Laser (Excitation), PMT, LP Mirror, BP Filter, Fluorochromes. Blue Laser 488nm.

BD Fortessa The BD Fortessa cell analyzer provides the flexibility to support measurement of multicolor flow cytometry assays of up to 16 parameters at a time. The FCF is equipped with one BD LSRFortessa™ (Diagnostic Purpose) and a BD LSRFortessa™ X-20 (Research Purpose). Breakthrough with the BD LSRFortessa™ X-20 Cell Analyzer. The BD LSRFortessa X-20 is a premium flow cytometer that brings endless possibilities to multicolor cell analysis. Designed for customization and optimized for reliability, the BD LSRFortessa family of cell analyzers allow you freedom of choice to achieve your research goals–your way. The BD LSR Fortessa™ X-20 cell analyzer delivers high-performance multicolor analysis detecting up to 18 parameters simultaneously with using 5 lasers: UV (355 nm) Violet (405 nm) Blue (488 nm) Y-G (561 nm) Red (640 nm) Fortessa X20 Flow Cytometer Quick guide Operation of the 5 laser Fortessa X20 BD Webinar CS&T System BD Compensation Guide… FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day. Fortessa behind right door above control panel) via the silicon tube to the sample needle and pull the syringe plunger.

Fisher Scientific and Jackson ImmunoResearch, 1:500). Cells were analysed on an LSR Fortessa and sorted with a BD Influx Cell Sorter (BD Biosciences).

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Instrument Systems (2) Application. Flow cytometry (2) Regulatory Status. RUO (2) Brand. BD LSRFortessa™ (2) Sort by: Instrument Systems (2 of 2) results found. BD LSRFortessa™ BD LSRFortessa Cell BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1.